Journal: Bio-protocol

Article Title: Measuring Electrophysiological Activity in Acute Brain Slices, Spheroids, and Organoids Using 3D High-Density Multielectrode Arrays

doi: 10.21769/BioProtoc.5708

Figure Lengend Snippet: (A) Use a cut-tip P200 pipette to harvest brain spheroids from a 96-well low adhesion plate into a Petri dish; a dark background helps visualize samples (top). Organoids are transferred from a Petri dish to a 1.7 mL tube by using a P1000 with the tip cut; no dark background is needed (bottom). (B) System setup: 1, 5%–10% carbon dioxide/air tank; 2, suction filtration flask; 3, mini-incubator box; 4, digital microscope mounted on its stand; 5, 3D HD-MEA locked into the BioCAM; 6, sample holder silicone net attached to the sample holder insert; 7, P200 or P1000 pipette with the tip cut; 8, spheroids/organoids are taken out from the incubator only during the mounting procedure. (C) Insert the sample holder frame into the 3D HD-MEA well (left), fill the reservoir with 2.7 mL of culture media, then place the samples on the recording area (middle). Finally, gently lower the sample holder insert to secure the spheroids/organoids in place (right).

Article Snippet: 5%–10% carbon dioxide/air tank (Air Liquide) 9.

Techniques: Transferring, Suction Filtration, Microscopy

Journal: Materials Today Bio

Article Title: Bioproduction of ∼10 knt single-stranded DNA for constructing large DNA origami structures

doi: 10.1016/j.mtbio.2026.103092

Figure Lengend Snippet: Schematic illustration of the biosynthesis of long ssDNA. The PCR-amplified dsDNA fragments were first assembled into recombinant phagemids using Gibson assembly and subsequently transformed into E. coli for amplification. This process was carried out in both shake-flask cultures and bioreactor systems, with systematic optimization of cultivation conditions to markedly enhance phage particle production. Finally, the resulting ssDNA was harvested and purified, and subsequently employed as a scaffold strand for the assembly of large-scale DNA origami nanostructures.

Article Snippet: Milligram-scale production of synthetic phage particles was carried out in a stirred-tank bioreactor (TMAXTREE Tmax Bio-3L) with a working volume of 1 L. XL1-Blue cells harboring the correctly assembled phagemid were grown to an OD 600 of 0.5, and 4 mL of this culture was inoculated into 2 × YT medium supplemented with 100 μg/mL ampicillin.

Techniques: Amplification, Recombinant, Transformation Assay, Purification

Journal: Materials Today Bio

Article Title: Bioproduction of ∼10 knt single-stranded DNA for constructing large DNA origami structures

doi: 10.1016/j.mtbio.2026.103092

Figure Lengend Snippet: Bioreactor production of 10,563 nt ssDNA. (a) Schematic illustration of ssDNA biosynthesis in a bioreactor system. (b) Growth curve of E. coli during bioreactor cultivation, showing cell density as a function of cultivation time. (c) Effect of bioreactor cultivation time on ssDNA yield, showing a maximum ssDNA production at 30 h of cultivation. All data are presented as mean ± SD (n = 3 biological replicates). Error bars represent standard deviations. (d) Comparison of ssDNA yields obtained using the bioreactor-based approach and reported methods .

Article Snippet: Milligram-scale production of synthetic phage particles was carried out in a stirred-tank bioreactor (TMAXTREE Tmax Bio-3L) with a working volume of 1 L. XL1-Blue cells harboring the correctly assembled phagemid were grown to an OD 600 of 0.5, and 4 mL of this culture was inoculated into 2 × YT medium supplemented with 100 μg/mL ampicillin.

Techniques: Comparison